Columbia University

Technology Ventures

Photochemical Methods for Covalent Attachment and Patterning Cross-linked and Self-Assembling Monolayer Surfaces

Technology #m05-091

Lead Inventors: Jeffrey T. Koberstein Ph.D., Nicholas J. Turro Ph.D., and Greg T. Carroll

Tech Ventures Reference: IRs M05-091 and M05-092

Problem or Unmet Need:
Surface modification, be it with polymers, proteins, or sugars, is of great interest, particularly for biological and chemical sensors. Some implementations rely on formation of a stable thin film, while others the attachment of specific molecules to interact with a particular target. Dewetting is a major problem for thin films, with most solutions employing complex polymer synthesis. Attachment of active molecules, such as carbohydrates, often requires modifications to the chemical structure of the molecule, potentially impairing its functionality. Thus a simple, reliable way to modify, and pattern surfaces while maintaining any inherent chemical functionality is needed.

Details of the Invention:
The technology described here is a chemical platform for modifying surfaces to tailor their physical and chemical properties. In one implementation a thin (10nm) layer of a photo-crosslinkable compound can be applied to a silicon substrate and upon exposure to ultraviolet (UV) irradiation it crosslinks forming a stable film, which has been shown to prevent dewetting. In another application, the photoactive monolayer can be formed on quartz, wafers or glass, and upon UV irradiation the monolayer can covalently bind carbohydrates directly. Further, the photoactive molecule can be incorporated into polymers and hydrogels. Both methods allow for spatially controlled patterning of the substrate with the use of a photomask. Additionally a micro-spotting technique can be used to pattern a carbohydrate micro-array by depositing small droplets of solution containing the desired sugar, limiting the region of attachment to the size of the droplet.

Applications:
• Scaffolds for biological materials
• Biological sensors
• Biomolecular array consumables for genomics, proteomics, and antigens
• Microstamps
• Glycoprotein proteomics and platform for screening antibody activity
• Photo-patterning of any biological or synthetic polymer containing C-H bond

Advantages:
• Surfaces can be patterned by photolithography or robotic spotter
• Sugar Arrays
o Any sugar can be attached without the need for any chemical modification
o Sugar modified surfaces are stable and robust
• Surface Wetting
o De novo polymer synthesis of thin film is not required
o Crosslinkable compound derivative readily available
o Clean, simple, and robust method to generate thin films


Patent Status: US Patent 8,158,832

Licensing Status: Available for Licensing and Sponsored Research Support

Publications:
Photons to illuminate the universe of sugar diversity through bioarrays; Gregory T. Carroll, Denong Wang, Nicholas J. Turro, Jeffrey T. Koberstein; Glycoconj J (2008) 25:5-10