Columbia University

Technology Ventures

Algorithm for detecting potential structural interactions among proteins

Technology #cu12266

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Researchers
Barry Honig
Managed By
Ron Katz

This technology is an algorithm that leverages genomic and structural information to detect potential interactions between proteins.

Unmet Need: Identifying potential protein structural interactions from sequence information

The identification of pairs of interacting proteins is essential for understanding cell regulatory mechanisms. However, most known protein-protein interactions have been deduced from exhaustive and time-consuming experimental studies such as the yeast two-hybrid assay, affinity purification, and manual curation. Additionally, while computational approaches have been developed to infer protein-protein interactions, they have failed to yield reliable genome-wide information.

The Technology: Algorithm identifies potential protein-protein interactions without time-consuming experimental studies

This technology is an algorithm that combines known information on the structure and sequence of proteins to predict new protein-protein interactions. Nicknamed PrePPI (predicting protein-protein interactions), this technology compares protein sequences to create structure-based sequence alignments that can be used to create interaction models between each pair of proteins in a dataset. These predicted protein pairs are then scored by the algorithm to yield the probability that the predicted interaction pair is true. By predicting the most likely protein-protein interactions, this technology bypasses the need for exhaustive experimentation, allowing researchers to selectively validate predicted pairs.

A benchmark study revealed that PrePPI provides superior information to that obtained from structural and non-structural evidence alone.

Applications:

  • Predicting protein-protein interactions
  • Tool for improved drug development
  • Research tool for determining protein interactomes

Advantages:

  • Improved protein-protein interaction predictions
  • Predicts interactions without time-consuming experimental studies
  • Outperforms related methods with lower false positive rate and higher true positive rate

Lead Inventor:

Barry Honig, Ph.D.

Related Publications:

Tech Ventures Reference:

  • IR CU12266

  • Licensing Contact: Ron Katz